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After inoculation and further growth, the inducer is often added in mid-log phase because the culture is growing fast and protein translation is maximal

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The National Institutes of Health recommend the following daily dosage of vitamin B12: Birth to 6 months: 0.4 micrograms Infants 712 months: 0.5 micrograms Children 13 years: 0.9 micrograms Children 48 years: 1.2 micrograms Children 913 years: 1.8 micrograms Teens 1418 years: 2.4 micrograms Adult men and women 1850 years: 2.4 micrograms Adult men and women over 50 years: 25250 micrograms Women who are pregnant: 2.6 micrograms Women who are breastfeeding: 2.8 micrograms Adults over the age of 50 are at a greater risk of vitamin B12 deficiency because they tend to produce less stomach acid, which is needed to convert the vitamin properly